Review

p atf2 (Cell Signaling Technology Inc)

Cell Signaling Technology Inc is a verified supplier

Cell Signaling Technology Inc manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Cell Signaling Technology Inc p atf2

Effect of transient SUMOylation inhibition in pre-adipocytes on cAMP-PKA-p38 signaling and adaptive thermogenesis in mature adipocytes. ( A ) Heatmap showing the stable upregulation of most genes involved in adaptive thermogenesis and cAMP-PKA-p38 signaling 22 days after adipogenic induction. Z -scores were calculated and plotted in GraphPad Prism. Treatments of pre-adipocytes were performed as shown in Fig. . ( B ) Western blot analysis of PKA substrates phosphorylation, assessed using a pan-PKA-target antibody, 22 days after adipogenic induction. TBP was used as a loading control. The asterisk (*) indicates substrates significantly affected by TAK-981 and/or rosiglitazone treatment. ( C ) Quantification of PKA substrate signals from panel (B). The signal for PKA substrates and TBP was quantified using Fiji software, with PKA substrate signals normalized to TBP. Error bars represent the standard deviation of four independent experiments. Student’s t -test was used to calculate P -values. ( D ) Western blot analysis of p-CREB, p-ATF1, p38, p-p38, and <t>p-ATF2,</t> 22 days after adipogenic induction. TBP was used as a loading control. ( E ) Quantification of p-CREB/ATF-1 signals from panel (D). Signals were normalized to TBP. Error bars represent the standard deviation of three independent experiments. Student’s t -test was used to calculate P -values. ( F ) Western blot analysis of p-CREB in the absence or presence of the PKA inhibitor RP-8-CPT-cAMPS. TBP was used as a loading control. Quantification of p-CREB, normalized to TBP, is showed in the lower panel. Error bars represent the standard deviation of two independent experiments. Quantification of p-p38 ( G ) and <t>p-ATF2</t> ( H ) from experiment in panel (D). Signals were normalized to TBP for p-ATF2 and to p38 for p-p38. Error bars represent the standard deviation of three independent experiments. Student’s t -test was used to calculate P -values.

P Atf2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+atf2/Phospho-ATF-2+(Thr71)%2FATF-7+(Thr53)+Antibody/pmc13000455-190-27-28
Average 92 stars, based on 56 article reviews

p atf2 - by Bioz Stars, 2026-09

92/100 stars

Images

1) Product Images from "Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fate"

Article Title: Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fate

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkag232

Figure Legend Snippet: Effect of transient SUMOylation inhibition in pre-adipocytes on cAMP-PKA-p38 signaling and adaptive thermogenesis in mature adipocytes. ( A ) Heatmap showing the stable upregulation of most genes involved in adaptive thermogenesis and cAMP-PKA-p38 signaling 22 days after adipogenic induction. Z -scores were calculated and plotted in GraphPad Prism. Treatments of pre-adipocytes were performed as shown in Fig. . ( B ) Western blot analysis of PKA substrates phosphorylation, assessed using a pan-PKA-target antibody, 22 days after adipogenic induction. TBP was used as a loading control. The asterisk (*) indicates substrates significantly affected by TAK-981 and/or rosiglitazone treatment. ( C ) Quantification of PKA substrate signals from panel (B). The signal for PKA substrates and TBP was quantified using Fiji software, with PKA substrate signals normalized to TBP. Error bars represent the standard deviation of four independent experiments. Student’s t -test was used to calculate P -values. ( D ) Western blot analysis of p-CREB, p-ATF1, p38, p-p38, and p-ATF2, 22 days after adipogenic induction. TBP was used as a loading control. ( E ) Quantification of p-CREB/ATF-1 signals from panel (D). Signals were normalized to TBP. Error bars represent the standard deviation of three independent experiments. Student’s t -test was used to calculate P -values. ( F ) Western blot analysis of p-CREB in the absence or presence of the PKA inhibitor RP-8-CPT-cAMPS. TBP was used as a loading control. Quantification of p-CREB, normalized to TBP, is showed in the lower panel. Error bars represent the standard deviation of two independent experiments. Quantification of p-p38 ( G ) and p-ATF2 ( H ) from experiment in panel (D). Signals were normalized to TBP for p-ATF2 and to p38 for p-p38. Error bars represent the standard deviation of three independent experiments. Student’s t -test was used to calculate P -values.

Techniques Used: Inhibition, Western Blot, Phospho-proteomics, Control, Software, Standard Deviation

Incubation:

Article Title: OGT-Mediated O-GlcNAcylation of ATF2 Protects Against Sepsis-Associated Encephalopathy by Inhibiting Microglial Pyroptosis.
Article Snippet: Microglial pyroptosis and neuroinflammation have been implicated in the pathogenesis of sepsis-associated encephalopathy (SAE).. OGT-mediated O-GlcNAcylation is involved in neurodevelopment and injury.. However, its regulatory function in microglial pyroptosis and involvement in SAE remains unclear.

Article Title: ATF2-LPCAT1-Mediated PKM2 Acetylation Links Cholesterol Stress to Macrophage Metabolic Reprogramming and Functional Remodeling
Article Snippet: Pyruvate kinase M2 (PKM2) regulates cellular metabolism under stress.. However, mechanisms involving PKM2 modification in cholesterol-loaded macrophages remain unclear.. In this study, we first characterized the impact of cholesterol loading on macrophage functional and metabolic alterations.

Article Title: Fibroblasts sense spatial proximity via an EGFR–CREB5 axis to restore quiescent synovial lining in remission rheumatoid arthritis
Article Snippet: Between 30-50 μg of protein was subjected to SDS-PAGE (Bio-Rad, #1610734) and transferred to a PVDF membrane (Bio-Rad, #1704273) using the BIO-Rad TRANS-BLOT TURBO transfer system (Bio-Rad, #690BR324) with a dry transfer protocol for 30 minutes according to the manufacturer’s instructions. .. Membranes were blocked for 15 minutes in Everyblot blocking buffer (Bio-Rad # 12010020) then incubated overnight at 4°C with primary antibodies against CREB5 (Proteintech, #14196-1-AP, 1:500 dilution), p-CREB (Cell Signaling Technology, #9198, 1:500), EGFR (Proteintech, # 66455-1-Ig),p-EGFR (Cell Signaling Technology, #3777), p-ATF2(Cell Signaling Technology, # 24329, 1:300), SOX5 (Proteintech, #13216-1-AP, 1:500), FOXO1 (Proteintech, #18592-1-AP, 1:500), EGFR (Proteintech, #66455-1-Ig), GAPDH (Thermo Fisher Scientific, #MA5-15738), ɑ-tubulin (11224-1-AP), or beta-actin (Cell Signaling Technology, #3700). .. Following primary antibody incubation, membranes were incubated with HRP-conjugated secondary antibodies (Thermo Fisher Scientific, anti-Rabbit #32460 or anti-Mouse #31430) for 1 hour at room temperature.

Blocking Assay:

Membrane:

Article Title: Microtubule Acetylation-Specific Inhibitors Induce Cell Death and Mitotic Arrest via JNK/AP-1 Activation in Triple-Negative Breast Cancer Cells
Article Snippet: Cells were lysed with radioimmunoprecipitation assay (RIPA) buffer composed of 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% NP-40, 0.1% SDS, 10 mM NaF, 1 mM Na3VO4, and protease inhibitor cocktail (Roche, Switzerland). .. The proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Merck, Germany) and detected using antibodies against acetyl-α-tubulin (T6199; Sigma), p-JNK (4668; Cell Signaling Technology [CST], USA), JNK (AHO1362; Invitrogen, USA), p-ERK (9106; CST), ERK (4696; CST), p-p38 (9215; CST), p38 (9212; CST), p-c-Jun (3270; CST), c-Jun (ab32137; Abcam, UK), c-Fos (2250; CST), p-ATF2 (27934; CST), ATF2 (35031; CST), Bcl2 (4223; CST), p-Histone H3 (9701; CST), p-cdc2 (4539; CST), p-Elk1 (9181; CST), α-tubulin (T6199; Sigma), and cyclin antibody sampler kit (9869; CST). .. Total RNA was isolated from cells using TRIzol (Invitrogen) according to the manufacturer’s protocol, and mRNA was reverse transcribed into cDNA using TOPscript RT DryMIX (Enzynomics, Korea).

Image Search Results

Journal: Nucleic Acids Research

Article Title: Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fate

doi: 10.1093/nar/gkag232

Figure Lengend Snippet: Effect of transient SUMOylation inhibition in pre-adipocytes on cAMP-PKA-p38 signaling and adaptive thermogenesis in mature adipocytes. ( A ) Heatmap showing the stable upregulation of most genes involved in adaptive thermogenesis and cAMP-PKA-p38 signaling 22 days after adipogenic induction. Z -scores were calculated and plotted in GraphPad Prism. Treatments of pre-adipocytes were performed as shown in Fig. . ( B ) Western blot analysis of PKA substrates phosphorylation, assessed using a pan-PKA-target antibody, 22 days after adipogenic induction. TBP was used as a loading control. The asterisk (*) indicates substrates significantly affected by TAK-981 and/or rosiglitazone treatment. ( C ) Quantification of PKA substrate signals from panel (B). The signal for PKA substrates and TBP was quantified using Fiji software, with PKA substrate signals normalized to TBP. Error bars represent the standard deviation of four independent experiments. Student’s t -test was used to calculate P -values. ( D ) Western blot analysis of p-CREB, p-ATF1, p38, p-p38, and p-ATF2, 22 days after adipogenic induction. TBP was used as a loading control. ( E ) Quantification of p-CREB/ATF-1 signals from panel (D). Signals were normalized to TBP. Error bars represent the standard deviation of three independent experiments. Student’s t -test was used to calculate P -values. ( F ) Western blot analysis of p-CREB in the absence or presence of the PKA inhibitor RP-8-CPT-cAMPS. TBP was used as a loading control. Quantification of p-CREB, normalized to TBP, is showed in the lower panel. Error bars represent the standard deviation of two independent experiments. Quantification of p-p38 ( G ) and p-ATF2 ( H ) from experiment in panel (D). Signals were normalized to TBP for p-ATF2 and to p38 for p-p38. Error bars represent the standard deviation of three independent experiments. Student’s t -test was used to calculate P -values.

Article Snippet: The following antibodies were used: UCP1, abcam, ab209483; PKA phospho-substrates, Cell Signaling, 9624; p-CREB/p-ATF1, Cell Signaling, 9198; p38 MAPK, Cell Signaling, 9212; p-p38 MAPK, Cell Signaling, 9211; p-ATF2, Cell Signaling, 24329; SUMO2/3, abcam, ab81371; PPARG, Cell signaling, 2443; and TBP, Protein Tech, 22006-1-AP or abcam, 282715; γ-tubulin, Sigma, T5326: CEBPB, Santa Cruz, sc-7962 quantifications were performed using FiJi [ ].

Techniques: Inhibition, Western Blot, Phospho-proteomics, Control, Software, Standard Deviation

The p38 MAPK activator LX-3 rescues the malignant behavior of HPSCC cells repressed by the silencing of THBS1 (A) Western blot analysis of the extent of p38, ATF2, and MK2 phosphorylation, as well as total p38 expression in KD-THBS1-treated HPSCC cells. (B) Western blot analysis of the extent of p38, ATF2, and MK2 phosphorylation, as well as total p38 expression in HPSCC cells treated with 2 μM LX-3 after knockdown of THBS1. (C–E) HPSCC cell proliferative capacity was examined using EdU staining. Transwell assay detects changes in the migratory (D) and invasive (E) abilities of HPSCC cells. (F) Expression of E-cadherin and N-cadherin in HPSCC cells was examined using dual-labeling immunofluorescence. The results are expressed as the mean ± SD (A–F). These values are derived from three independent experiments. Statistical analysis was performed using an unpaired t test.

Journal: iScience

Article Title: THBS1 upregulation by KLF7 in hypopharyngeal squamous cell carcinoma contributes to lung metastasis through the p38 MAPK signaling

doi: 10.1016/j.isci.2026.114816

Figure Lengend Snippet: The p38 MAPK activator LX-3 rescues the malignant behavior of HPSCC cells repressed by the silencing of THBS1 (A) Western blot analysis of the extent of p38, ATF2, and MK2 phosphorylation, as well as total p38 expression in KD-THBS1-treated HPSCC cells. (B) Western blot analysis of the extent of p38, ATF2, and MK2 phosphorylation, as well as total p38 expression in HPSCC cells treated with 2 μM LX-3 after knockdown of THBS1. (C–E) HPSCC cell proliferative capacity was examined using EdU staining. Transwell assay detects changes in the migratory (D) and invasive (E) abilities of HPSCC cells. (F) Expression of E-cadherin and N-cadherin in HPSCC cells was examined using dual-labeling immunofluorescence. The results are expressed as the mean ± SD (A–F). These values are derived from three independent experiments. Statistical analysis was performed using an unpaired t test.

Article Snippet: The primary antibodies used were as follows: anti-THBS1 (1:250, A2125, BioAb), anti-E-cadherin (1:1000, 3195, Cell Signaling Technologies), anti-N-cadherin (1:100, PA5-19486m Invitrogen), anti-Phospho-p38 MAPK (Thr180/Tyr182) (1:1000, 4511, Cell Signaling Technologies), anti-total p38 (1:1000, 9212, Cell Signaling Technologies), anti- p -ATF2 (Thr71) (1:1000, 44-295G, Invitrogen), anti-p-MK2 (Thr334) (1:1000, 3007, Cell Signaling Technologies), anti-KLF7 (1:1000, CSB- PA126129 , Cusabio), anti-Slug (1:1000, 12129-1-AP, ProteinTech Group), anti-Vimentin (1:20000, 10366-1-AP, ProteinTech Group), anti-HDAC6 (1:1000, 12834-1-AP, ProteinTech Group), and anti-GAPDH (1:20000, AC001, BioAb).

Techniques: Western Blot, Phospho-proteomics, Expressing, Knockdown, Staining, Transwell Assay, Labeling, Immunofluorescence, Derivative Assay

Reactivation of THBS1 rescues the HPSCC cell malignant biological behavior curtailed by knockdown of KLF7 (A) Western blot analysis of protein expression of KLF7 and THBS1 in HPSCC cells treated with knockdown of KLF7 combined with the overexpression of THBS1. (B–D) HPSCC cell proliferative capacity was examined using EdU staining. Transwell assay detects changes in the migratory (C) and invasive (D) abilities of HPSCC cells. (E) Expression of E-cadherin and N-cadherin in HPSCC cells was examined using dual-labeling immunofluorescence. (F) Western blot analysis of the extent of p38, ATF2, and MK2 phosphorylation, as well as total p38 expression in HPSCC cells. The results are expressed as the mean ± SD (A–F). These values are derived from three independent experiments. Statistical analysis was performed using a one-way ANOVA followed by Tukey’s multiple comparison test.

Journal: iScience

Article Title: THBS1 upregulation by KLF7 in hypopharyngeal squamous cell carcinoma contributes to lung metastasis through the p38 MAPK signaling

doi: 10.1016/j.isci.2026.114816

Figure Lengend Snippet: Reactivation of THBS1 rescues the HPSCC cell malignant biological behavior curtailed by knockdown of KLF7 (A) Western blot analysis of protein expression of KLF7 and THBS1 in HPSCC cells treated with knockdown of KLF7 combined with the overexpression of THBS1. (B–D) HPSCC cell proliferative capacity was examined using EdU staining. Transwell assay detects changes in the migratory (C) and invasive (D) abilities of HPSCC cells. (E) Expression of E-cadherin and N-cadherin in HPSCC cells was examined using dual-labeling immunofluorescence. (F) Western blot analysis of the extent of p38, ATF2, and MK2 phosphorylation, as well as total p38 expression in HPSCC cells. The results are expressed as the mean ± SD (A–F). These values are derived from three independent experiments. Statistical analysis was performed using a one-way ANOVA followed by Tukey’s multiple comparison test.

Techniques: Knockdown, Western Blot, Expressing, Over Expression, Staining, Transwell Assay, Labeling, Immunofluorescence, Phospho-proteomics, Derivative Assay, Comparison

Activation of the THBS1/p38 MAPK axis by KLF7 promotes the lung metastasis of HPSCC cells (A) Lung metastasis in nude mice injected with FaDu cells in the tail vein was assessed by HE staining. (B–D) Quantitative results of lung metastatic nodules. IHC scores (C) and protein expression (D) of Slug and Vimentin within lung metastases in nude mice. (E) RT-qPCR detection of mRNA expression levels of KLF7 and THBS1 in lung metastases. (F) IHC scores of KLF7 and THBS1 within lung metastases in nude mice. (G) Western blot analysis of the extent of p38, ATF2, and MK2 phosphorylation, as well as total p38 expression in lung metastases. The results are expressed as the mean ± SD (A–G). These values are derived from five mice. Statistical analysis was performed using a one-way ANOVA followed by Tukey’s multiple comparison test.

Journal: iScience

Article Title: THBS1 upregulation by KLF7 in hypopharyngeal squamous cell carcinoma contributes to lung metastasis through the p38 MAPK signaling

doi: 10.1016/j.isci.2026.114816

Figure Lengend Snippet: Activation of the THBS1/p38 MAPK axis by KLF7 promotes the lung metastasis of HPSCC cells (A) Lung metastasis in nude mice injected with FaDu cells in the tail vein was assessed by HE staining. (B–D) Quantitative results of lung metastatic nodules. IHC scores (C) and protein expression (D) of Slug and Vimentin within lung metastases in nude mice. (E) RT-qPCR detection of mRNA expression levels of KLF7 and THBS1 in lung metastases. (F) IHC scores of KLF7 and THBS1 within lung metastases in nude mice. (G) Western blot analysis of the extent of p38, ATF2, and MK2 phosphorylation, as well as total p38 expression in lung metastases. The results are expressed as the mean ± SD (A–G). These values are derived from five mice. Statistical analysis was performed using a one-way ANOVA followed by Tukey’s multiple comparison test.

Techniques: Activation Assay, Injection, Staining, Expressing, Quantitative RT-PCR, Western Blot, Phospho-proteomics, Derivative Assay, Comparison

Review

p atf2 (Cell Signaling Technology Inc)

Structured Review

Images

1) Product Images from "Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fate"

Related Articles

Incubation:

Blocking Assay:

Membrane:

Similar Products

Image Search Results